Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Circulating circRNAs expression landscape of in CRC, CRA, and healthy control samples. (A and B) Cluster analysis of differentially expressed circRNA in each group. (C) Volcano blot presented differentially expressed circRNA. (D) Intersection matching analysis of CRA group and healthy control compared with CRC group. CRA, colorectal adenomas; CRC, colorectal cancer

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing, Control

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Relative expression of circRNA in training set. The eight circRNAs were examined in 20 paired samples of three group. Data was presented as mean ± SD, **indicated p < 0.01. *indicated p < 0.05, n.s, indicated no significance

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Relative expression of candidate circRNA in independent cohort. The eight circRNAs were examined in 80 paired plasma samples from healthy controls, CRA patients and CRC patients. **indicated p < 0.01. *indicated p < 0.05, n.s, indicated no significance

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing, Clinical Proteomics

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: CircRNA panel predicted CRC from healthy controls. (A) Diagnostic efficacy of three circRNA panel as diagnostic marker for colorectal cancer in healthy population in training sets. (B) Diagnostic efficacy of three circRNA panel as biomarker of colorectal cancer in healthy population in validation set

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Diagnostic Assay, Marker, Biomarker Discovery

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: CircRNA panel predicted CRC from CRA. (A) Diagnostic efficacy of three circRNA panel as diagnostic marker for colorectal cancer in CRA patients in training sets. (B) Diagnostic efficacy of three circRNA panel as biomarker of colorectal cancer in CRA patients in validation set

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Diagnostic Assay, Marker, Biomarker Discovery

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap of microarray data showing differential expression of circRNAs between malignant, benign and normal cell lines. Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3). circRNAs were more likely to be down-regulated in normal and benign cell lines compared to malignant cells.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Microarray, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in PCa (malignant vs. normal/benign cell lines)*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap showing differential expression of circRNAs between AR dependent cells LNCaP, 22Rv1 and VCaP (hormone sensitive) and AR independent cells DU145 and PC-3 (castration resistant). Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3).

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 up-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: Comparison of circRNA expression profiles between HPA-v and adipocytes. (A) Box plots revealed the distribution of circRNAs in the six samples after normalization. (B) Volcano plots revealed the differentially expressed circRNAs. Green and red dots represent significantly down- and upregulated circRNAs in adipocytes compared with HPA-v, respectively (fold change ≥5.0, P<0.01). (C) Hierarchical clustering was performed to reveal the differentially expressed circRNAs between HPA-v and adipocytes. (D) Expression patterns of select differentially expressed circRNAs in HPA-v and adipocytes were determined by qPCR. (E) The heatmap revealed the selected differentially expressed circRNAs in HPA-v and adipocytes. *P<0.05. circRNA, circular RNA; HPA-v, human preadipocytes from visceral fat tissue; AD, adipocytes; n.d., not detected.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: Top 10 up- and downregulated circRNAs in adipocytes.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: The top 15 significantly enriched pathways associated with the differentially expressed circRNA parental genes according to KEGG analysis. circRNAs, circular RNAs; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: miRNAs with >2 miRNA response elements targeting the top 10 upregulated and downregulated circRNAs.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Molecular Medicine Reports

Article Title: A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR-101b by targeting TGFβRI

doi: 10.3892/mmr.2020.11486

Figure Lengend Snippet: circRNA microarray analysis and circRNA_0000491 are upregulated in db/db diabetic nephropathy mice kidney tissues and MES13 cells treated with high glucose. (A) Heat maps and (B) volcano plots displaying the differentially expressed circRNAs. (C) A schematic diagram of the genomic location and structure of circ_0000491. circRNA, circular RNA.

Article Snippet: Subsequently, total RNA was extracted from the kidney cortex using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and RNA purity, concentration and integrity were measured using a NanoPhotometer ® spectrophotometer (IMPLEN), a Qubit ® RNA assay kit in the Qubit ® 2.0 Flurometer (Thermo Fisher Scientific, Inc.) and an RNA Nano 6000 Assay kit in the Bioanalyzer 2100 system (Agilent Technologies, Inc.), respectively. circRNA microarray analysis was performed by Novogene Co., Ltd.

Techniques: Microarray

Journal: Molecular Medicine Reports

Article Title: A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR-101b by targeting TGFβRI

doi: 10.3892/mmr.2020.11486

Figure Lengend Snippet: Expression of circRNA_0000491 in the kidney tissue of db/db mice and high glucose-induced mouse MES13 cells. (A) Reverse transcription-quantitative PCR estimated the expression levels of circ_0000491 in diabetic nephropathy mice and (B) the high concentration glucose (30 mM)-induced MES13 cells. ## P<0.01 vs. the db/m control group; **P<0.01 vs. the 5.5 mM glucose treated MES13 cells group. circRNA, circular RNA.

Article Snippet: Subsequently, total RNA was extracted from the kidney cortex using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and RNA purity, concentration and integrity were measured using a NanoPhotometer ® spectrophotometer (IMPLEN), a Qubit ® RNA assay kit in the Qubit ® 2.0 Flurometer (Thermo Fisher Scientific, Inc.) and an RNA Nano 6000 Assay kit in the Bioanalyzer 2100 system (Agilent Technologies, Inc.), respectively. circRNA microarray analysis was performed by Novogene Co., Ltd.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Concentration Assay, Control

Journal: Molecular Medicine Reports

Article Title: A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR-101b by targeting TGFβRI

doi: 10.3892/mmr.2020.11486

Figure Lengend Snippet: Circ_0000491 silencing inhibits the extracellular matrix accumulation and fibrosis-associated protein synthesis in MES13 cells. RT-qPCR revealed the expression levels of E-cad, Vim, FN, α-SMA, Col. I, Col. III and Col. IV in (A) diabetic nephropathy mice and (B) MES13 cells treated with high glucose. (C) The expression levels of circ_0000491 in MES13 cells transfected using siRNA. (D) Western blotting showing the protein expression levels of (E) E-cad, (F) Vim, (G) FN, (H) α-SMA, (I) Col. I, (J) Col. III and (K) Col. IV in MES13 cells with high glucose treatment and transfected with si_circ_0000491. *P<0.05, **P<0.01 vs. the corresponding control group; # P<0.05, ## P<0.01 vs. the Si_Circ_NC group. Col., collagen; circRNA, circular RNA; E-cad, E-cadherin; FN, fibronectin; si, small interfering; SMA, smooth muscle actin; Vim, vimentin.

Article Snippet: Subsequently, total RNA was extracted from the kidney cortex using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and RNA purity, concentration and integrity were measured using a NanoPhotometer ® spectrophotometer (IMPLEN), a Qubit ® RNA assay kit in the Qubit ® 2.0 Flurometer (Thermo Fisher Scientific, Inc.) and an RNA Nano 6000 Assay kit in the Bioanalyzer 2100 system (Agilent Technologies, Inc.), respectively. circRNA microarray analysis was performed by Novogene Co., Ltd.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Western Blot, Control

Journal: Molecular Medicine Reports

Article Title: A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR-101b by targeting TGFβRI

doi: 10.3892/mmr.2020.11486

Figure Lengend Snippet: Circ_0000491 targets miRNA-101b. (A) The miR-101b mRNA expression levels in MES13 cells transfected with miR-101b mimics or miR-101b inhibitor was confirmed using RT-qPCR. (B) Circ_0000491 expression was examined in the transfected MES13 cells. (C) Diagram of the miR-101b putative binding sites in circ_0000491. (D) miR-101b expression in db/db mice and high glucose treated MES13 cells was measured using RT-qPCR. (E) Luciferase reporter assay showed the binding activity of miR-101b and circRNA_0000491. (F) miR-101b mRNA expression levels in MES13 cells transfected with si_circ_0000491 was measured using RT-qPCR. *P<0.05, **P<0.01 vs. the corresponding control group. circRNA, circular RNA; miR/miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering.

Article Snippet: Subsequently, total RNA was extracted from the kidney cortex using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and RNA purity, concentration and integrity were measured using a NanoPhotometer ® spectrophotometer (IMPLEN), a Qubit ® RNA assay kit in the Qubit ® 2.0 Flurometer (Thermo Fisher Scientific, Inc.) and an RNA Nano 6000 Assay kit in the Bioanalyzer 2100 system (Agilent Technologies, Inc.), respectively. circRNA microarray analysis was performed by Novogene Co., Ltd.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR-101b by targeting TGFβRI

doi: 10.3892/mmr.2020.11486

Figure Lengend Snippet: Effect of circ_0000491 and miRNA-101b inhibition on extracellular matrix and fibrosis-associated protein expression via targeting TGFβRI. (A) Western blotting showed that miR-101b inhibitor reversed the effect of si_circRNA_0000491 on the protein expression levels of (B) TGFβ1, (C) TGFβRI, (D) pSmad3/Smad3, (E) E-cad, (F) Vim, (G) FN, (H) α-SMA, (I) Col. I, (J) Col. III and (K) Col. IV. *P<0.05, **P<0.01. Col., collagen; circRNA, circular RNA; E-cad, E-cadherin; FN, fibronectin; miR/miRNA, microRNA; p, phosphorylated; si, small interfering; SMA, smooth muscle actin; TGFβR1, TGFβ receptor 1; Vim, vimentin.

Article Snippet: Subsequently, total RNA was extracted from the kidney cortex using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), and RNA purity, concentration and integrity were measured using a NanoPhotometer ® spectrophotometer (IMPLEN), a Qubit ® RNA assay kit in the Qubit ® 2.0 Flurometer (Thermo Fisher Scientific, Inc.) and an RNA Nano 6000 Assay kit in the Bioanalyzer 2100 system (Agilent Technologies, Inc.), respectively. circRNA microarray analysis was performed by Novogene Co., Ltd.

Techniques: Inhibition, Expressing, Western Blot